Culture Collections

General Cell Collection Detail

ECACC General Cell Collection: HCA-46

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 07031601
Cell Line Name: HCA-46
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: HCA-46 (ECACC 07031601)
Keywords: Human colon adenocarcinoma
Cell Line Description: Established from a sigmoid colon adenocarcinoma of a 53 year-old female patient. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.
Species: Human
Tissue of Origin: Colon
CellType: Epithelial
Growth Mode: Adherent
DNA Profile: STR-PCR Data:

Amelogenin: X, X
CSF1PO: 11,12
D13S317: 10,11
D16S539: 11,13
D5S818: 11,12
D7S820: 10,14
THO1: 6,9.3
TPOX: 8,9
vWA: 17

Karyotype: Not specified
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.05% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm² when splitting.  Seed at approx 6 x 10,000 cells/cm² when resuscitating from frozen.  Following resuscitation or subculture the cells may take at least 48 hours to re-attach. They should be left without disturbance during this time to facilitate adhesion. These cells may take up to one week from resuscitation to establish and will require media changes during this time. The cells grow in discrete islands and do not form a monolayer within the flask. Cell counts should be performed at each subculture to monitor cell proliferation.
Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Depositor: Dr S Kirkland, Department of Histopathology, Imperial College London, Hammersmith Campus, DuCane Road, London, W12 0NN
Originator: Yes
Country: UK
References: S. C. Kirkland and I. G. Bailey. (1986) Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer, 53, 6, 779-85.
Additional Bibliography: P53 DeletionY. Liu and W.F. Bodmer (2006)Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines.PNAS 103, 976-981.Beta-catenin MutationM. Ilyas, I.P.M. Tomlinson, A. Rowan, M. Pignatelli and W.F. Bodmer (1997)Beta-catenin mutations in cell lines established from human colorectal cancers.PNAS 94, 10330-10334.
Patents: None Specified By Depositor
Release Conditions: Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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