An area of regulatory concern in the biopharmaceutical production industry is the use of animal derived products during production processes. One of the sources of this problem is the presence of animal derived components in the media (e.g. serum and growth factors) used to grow cell lines which produce the required product.
For years media manufacturers have produced media designed to support the growth of cell lines without the addition of serum and more recently in completely animal component-free/chemically defined media (Sigma-Aldrich) even to the extent of being peptide-free (Cell Culture Technologies). The lengthy adaptation process before such a media can be incorporated in a production process along with the unreliability of the early serum-free media have often been a barrier to change. For this reason and due to customer demand ECACC has been working with media suppliers to provide a range of common cell lines which have already been adapted to growth in serum and protein free media.
In addition to the regulatory concerns mentioned above removal of serum from cell culture offers many other advantages such as:
The table below lists cell lines currently available from ECACC adapted to growth in serum/protein free medium.
|Catalogue No.||Cell Line Name||Keywords|
|06020202||NS0-Turbodoma||Mouse myeloma, serum-free, without cholesterol|
|03061601||NS0-Serum-Free||Mouse myeloma, serum-free|
|00102307||CHO (PROTEIN FREE)||Chinese hamster ovary|
|02050101||MDCK-Protein Free||Canine Cocker Spaniel kidney|
|03092503||Vero-Hektor||African Green Monkey kidney cells, serum-free|
|87032401||L-M||Mouse C34/An connective tissue|
|03092501||Sp2/0-Ag14-TurboDoma||Mouse x Mouse hybridoma, non-producing, serum-free|
|03092502||P3X63Ag8.653-TurboDoma||Mouse BALB/c myeloma, serum-free|
|04092902||COS-1-InVitrus||Monkey African green kidney, SV40 transformed, serum-free|
|05062302||BHK21-InVitrus||Hamster Syrian kidney, serum-free|
|05063001||COS-7-InVitrus||Monkey African green kidney, SV40 transformed, serum-free|
|05011003||293 GTP-AC-free||Human Embryo Kidney, serum-free|
|05011001||Sf9 TiterHigh AC free||Spodoptera frugiperda pupal ovarian tissue, serum-free|
|05030202||Sf21 TiterHigh AC free||Spodoptera frugiperda pupal ovarian tissue, serum-free|
|05011002||CHO/dhFr-AC-free||Hamster Chinese Ovary, serum-free|
|05062301||BHK21-Hektor||Hamster Syrian kidney, serum-free|
|10021102||Bob||Human prostate cancer spontaneously immortalized, serum-free|
|07052901||PNT1A (SERUM FREE)||Human post pubertal normal, immortalised with SV40, serum-free|
|07042701||PNT2 (SERUM FREE)||Human prostate normal, serum-free|
|08060101||Sp2/0-Ag14 (serum-free)||Mouse x mouse hybridoma non-secreting, serum-free, animal component (AC) free|
|Cell Line Name||Catalogue No.||Reactivity||Antibody Isotype|
|HyGPD-YK-1-1||92120704||Anti- muscle glyceraldehyde-3-phosphate dehydrogenase, rabbit||IgG2b|
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